OrangerV1, Main, Exploration, bibRecord, 002B26

Regulation of gibberellin 20-oxidase gene expression and gibberellin content in citrus by temperature and citrus exocortis viroid

Identifieur interne : 002B26 ( Main/Exploration ); précédent : 002B25; suivant : 002B27

Regulation of gibberellin 20-oxidase gene expression and gibberellin content in citrus by temperature and citrus exocortis viroid

Auteurs : Ana M. Vidal [Espagne] ; Waddi Ben-Cheikh [Espagne] ; Manuel Talon [Espagne] ; José L. Garcia-Martinez [Espagne]

Source :

Planta [ 0032-0935 ] ; 2003.

RBID : Pascal:03-0411430

Descripteurs français

Pascal (Inist)
- Gibbérelline, Biosynthèse, Expression génique, Gène, Température, Croissance, Citrus, Viroïde exocortis agrume, GA 20-oxidase.

English descriptors

KwdEn :
- Biosynthesis, Citrus, Citrus exocortis viroid, Gene, Gene expression, Gibberellin, Growth, Temperature.

Abstract

A cDNA clone coding for a gibberellin (GA) 20-oxidase (CcGA20oxl), an enzyme of GA biosynthesis, which when expressed in vitro catalyzed the conversion of GA₁₂ to GA₉ and of GA₅₃ to GA₂₀, was isolated from the citrus hybrid Carrizo citrange (Citrus sinensis x Poncirus trifoliata). Transcripts of CcGA20ox1 were abundant in the apex and leaves and much less abundant in internodes, nodes and roots. Seedlings of Carrizo citrange cultured under a 32 °C/27 °C (day/ night) regime elongated more than seedlings growing under 17°C/12°C conditions. The effect of higher temperature was associated with more CcGA20oxl transcripts and with higher content of GA₁, the main active GA in citrus, in the shoot. The infection of Etrog citron (Citrus medica) plants with citrus exocortis viroid (CEVd), which produces a stunted phenotype, reduced the levels of transcripts in the apical shoot hybridizing to the gene CcGA20oxl of Carrizo citrange and the content of GA₁. Thus GA₁ content correlated with CcGA20oxl transcript levels. In contrast, results for gibberellic acid (GAS) and paclobutrazol applications to Carrizo citrange showed that CcGA200oxl expression was subject to feed-back regulation. These observations indicate that the feed-back regulation of GA20ox operates mostly when the levels of active GAs have been dramatically altered. The results also show that the growth reduction induced by environmental (temperature) and biotic (CEVd) factors may be partially due to the modulation of the expression of GA200ox genes.

Affiliations:

Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: 000742
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to stream Main, to step Curation: 002B26

Le document en format XML

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<author><name sortKey="Garcia Martinez, Jose L" sort="Garcia Martinez, Jose L" uniqKey="Garcia Martinez J" first="José L." last="Garcia-Martinez">José L. Garcia-Martinez</name>
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<affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-CSIC, Avda de los Naranjos s/n</s1>
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<country>Espagne</country>
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<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Instituto Valenciano de Investigaciones Agrarias</s1>
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<sZ>1 aut.</sZ>
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<country>Espagne</country>
<wicri:noRegion>Instituto Valenciano de Investigaciones Agrarias</wicri:noRegion>
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<author><name sortKey="Ben Cheikh, Waddi" sort="Ben Cheikh, Waddi" uniqKey="Ben Cheikh W" first="Waddi" last="Ben-Cheikh">Waddi Ben-Cheikh</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Instituto Valenciano de Investigaciones Agrarias</s1>
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<country>Espagne</country>
<wicri:noRegion>Instituto Valenciano de Investigaciones Agrarias</wicri:noRegion>
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<author><name sortKey="Talon, Manuel" sort="Talon, Manuel" uniqKey="Talon M" first="Manuel" last="Talon">Manuel Talon</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Instituto Valenciano de Investigaciones Agrarias</s1>
<s2>46113 Montcada, Valencia</s2>
<s3>ESP</s3>
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<country>Espagne</country>
<wicri:noRegion>Instituto Valenciano de Investigaciones Agrarias</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Garcia Martinez, Jose L" sort="Garcia Martinez, Jose L" uniqKey="Garcia Martinez J" first="José L." last="Garcia-Martinez">José L. Garcia-Martinez</name>
<affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-CSIC, Avda de los Naranjos s/n</s1>
<s2>46022 Valencia</s2>
<s3>ESP</s3>
<sZ>1 aut.</sZ>
<sZ>4 aut.</sZ>
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<country>Espagne</country>
<placeName><region nuts="2" type="communauté">Communauté valencienne</region>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biosynthesis</term>
<term>Citrus</term>
<term>Citrus exocortis viroid</term>
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<term>Gene expression</term>
<term>Gibberellin</term>
<term>Growth</term>
<term>Temperature</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Gibbérelline</term>
<term>Biosynthèse</term>
<term>Expression génique</term>
<term>Gène</term>
<term>Température</term>
<term>Croissance</term>
<term>Citrus</term>
<term>Viroïde exocortis agrume</term>
<term>GA 20-oxidase</term>
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<front><div type="abstract" xml:lang="en">A cDNA clone coding for a gibberellin (GA) 20-oxidase (CcGA20oxl), an enzyme of GA biosynthesis, which when expressed in vitro catalyzed the conversion of GA<sub>12</sub>
 to GA<sub>9</sub>
 and of GA<sub>53</sub>
 to GA<sub>20</sub>
, was isolated from the citrus hybrid Carrizo citrange (Citrus sinensis x Poncirus trifoliata). Transcripts of CcGA20ox1 were abundant in the apex and leaves and much less abundant in internodes, nodes and roots. Seedlings of Carrizo citrange cultured under a 32 °C/27 °C (day/ night) regime elongated more than seedlings growing under 17°C/12°C conditions. The effect of higher temperature was associated with more CcGA20oxl transcripts and with higher content of GA<sub>1</sub>
, the main active GA in citrus, in the shoot. The infection of Etrog citron (Citrus medica) plants with citrus exocortis viroid (CEVd), which produces a stunted phenotype, reduced the levels of transcripts in the apical shoot hybridizing to the gene CcGA20oxl of Carrizo citrange and the content of GA<sub>1</sub>
. Thus GA<sub>1</sub>
 content correlated with CcGA20oxl transcript levels. In contrast, results for gibberellic acid (GAS) and paclobutrazol applications to Carrizo citrange showed that CcGA200oxl expression was subject to feed-back regulation. These observations indicate that the feed-back regulation of GA20ox operates mostly when the levels of active GAs have been dramatically altered. The results also show that the growth reduction induced by environmental (temperature) and biotic (CEVd) factors may be partially due to the modulation of the expression of GA200ox genes.</div>
</front>
</TEI>
<affiliations><list><country><li>Espagne</li>
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<tree><country name="Espagne"><region name="Communauté valencienne"><name sortKey="Vidal, Ana M" sort="Vidal, Ana M" uniqKey="Vidal A" first="Ana M." last="Vidal">Ana M. Vidal</name>
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<name sortKey="Ben Cheikh, Waddi" sort="Ben Cheikh, Waddi" uniqKey="Ben Cheikh W" first="Waddi" last="Ben-Cheikh">Waddi Ben-Cheikh</name>
<name sortKey="Garcia Martinez, Jose L" sort="Garcia Martinez, Jose L" uniqKey="Garcia Martinez J" first="José L." last="Garcia-Martinez">José L. Garcia-Martinez</name>
<name sortKey="Talon, Manuel" sort="Talon, Manuel" uniqKey="Talon M" first="Manuel" last="Talon">Manuel Talon</name>
<name sortKey="Vidal, Ana M" sort="Vidal, Ana M" uniqKey="Vidal A" first="Ana M." last="Vidal">Ana M. Vidal</name>
</country>
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Regulation of gibberellin 20-oxidase gene expression and gibberellin content in citrus by temperature and citrus exocortis viroid

Regulation of gibberellin 20-oxidase gene expression and gibberellin content in citrus by temperature and citrus exocortis viroid

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri